P 23 - Efficacy evaluation of Natular^® DT larvicide in the eastern Amazon region of Brazil

The Aedes aegypti mosquito is associated to important arboviruses including Dengue, Zika and Chikungunya. Mosquito control is a goal of most countries present in the species' distribution range, given the public health problems caused to the population. In Brazil, biolarvicides’ evalution is important to increase control alternatives existing and used in the country. This study evaluated the effectiveness of the Natular® DT product in field simulation tests and directly in the field, in the city of Macapá, Amapá, Brazil. The field simulation was conducted in plastic tanks containing 40 liters of water in shaded and exposed to the sun areas, with 60% water exchange weekly and using Ae. aegypti larvae from Rockefeller lineage and a local population. For the field evaluation, a neighborhood area of Macapá was selected and the product was used following the municipality vector control program methodology. In this area, the treated deposits were labeled and identified to track positivity. Another area was maintained in the program routine using pyriproxyfen and considered a control area. Two intervention cycles were used, lasting two months each, with evaluation of methodologies using ovitrap types. In the field simulation, mortality above 80% was observed for up to 42 days, and larvae was detected in the treated and labeled deposits after 42 days of treatment. Areas where the Natular DT was used, the ovitraps showed that the density of Aedes eggs was reduced during the study period but increased at the end of the assessment. The residuality presented by Natular® DT may vary depending on the location where the product is used, due to the complexity of breeders in each location. The results showed that this product can be applied in control actions but highlights the importance of mechanical control and health education activities to the population.