Session: Collaboration and Innovation: CDCs COE Partnerships with AMCA Professionals Symposium I
213 - Collaborating with Vector Control to explore the connection between laboratory resistance assays and control efficacy for Culex tarsalis
Thursday, March 7, 2024
8:50am – 9:10am
Location: A2
Abstract: One of the research priorities for the Centers of Excellence was to evaluate the impact of insecticide resistance on operational mosquito control.To approach this priority, the Thiemann Lab at University of the Pacific and the Sacramento-Yolo Mosquito and Vector Control District are collaborating on a project to make connections between laboratory resistance assays, semi-field cage trials, and changes in abundance that result from insecticide application. The first insecticide tested was a natural pyrethrin. Culex tarsalis, from the same population in Natomas, CA, were used in standard bottle bioassays with and without piperonyl butoxide (120-minute mortality and median lethal time), topical applications (LD50s), and a novel individual bottle bioassay (individual knockdown time).The results from these assays were compared to detoxifying enzyme levels, as well as kdr prevalence, in the population.In addition to the laboratory work, mosquitoes from the same population were treated with Pyronyl 525 Oil Concentrate [active ingredients: 5% pyrethrins, 25% piperonyl butoxide] in cage trials (evaluating percent mortality) and field trials (evaluating abundance reduction).Analysis is ongoing to explore the relationship between susceptibility, as measured in the lab, and operational success in the field.
