264 - Considerations for assessing methoprene bioefficacy in the lab and in the field

Insecticide resistance monitoring is crucial to the success of integrated vector management programs. Methoprene, as a biorational insect growth regulator has fulfilled a significant role in the management and control of mosquitoes for nearly half a century and continues to do so. The availability and flexibility of methoprene formulations coupled with wide-ranging larval habitats and delivery platforms necessitate sound methodology to assess bioefficacy and detect tolerance or resistance in a variety of mosquito species. Here, we present key considerations for the assessment of methoprene bioefficacy in the lab and in the field. We will discuss important aspects of laboratory-based bioassays for methoprene, to include appropriate materials, larval life-stages, and source of active ingredient. In addition, we will frame laboratory testing in the context of integrated measures of efficacy which should include field validation methods such as assessing habitat, extraction of pupae and determining inhibition of adult emergence.