254 - Statewide examination of insecticide resistance and mechanisms in Florida Culex quinquefasciatus
Thursday, March 7, 2024
2:35pm – 2:45pm
Location: D2
Abstract: Culex quinquefasciatus serves as a primary vector of West Nile virus and St. Louis encephalitis viruses in Florida. Only limited insecticide resistance testing of Florida Cx. quinquefasciatus populations has been previously performed; therefore, we undertook a comprehensive, statewide evaluation of pyrethroid and organophosphate phenotypic insecticide resistance and the potential mechanisms responsible. We found that all Florida Cx. quinquefasciatus populations tested were resistant to pyrethroids but varied in susceptibility to organophosphates. While the L1014F conversion and kdr-associated resistance was identified in many Florida Cx. quinquefasciatus, detoxification enzyme-based resistance primarily mediated by beta-esterases appeared to play a more important role in the phenotypic resistance we observed in CDC bottle bioassays.