Abstract: Bridge vectors have the potential to harbor and transmit pathogens outside the typical range of their primary vectors, and therefore are not traditionally targeted in evaluation of control efforts. Furthermore, a lack of lab based colonies to support insecticide evaluation through the use of bioassays renders them less targeted. Here we present the methods to colonize Psorophora ferox, a bridge vector, from a wild population from Gainesville, FL, and the bionomics of this species. We also discuss potential projects with this bridge vector to help understand other target/primary vectors of interest.